Advertisement

Contact Person

Dr. Elinor Switzer

Managing Editor

Phone: +49 (0)711 - 2 29 87 63
Fax: +49 (0)711 - 2 29 87 65
send an Email


Archive

Impact of dabigatran on a large panel of routine or specific coagulation assays

Journal: Thrombosis and Haemostasis
ISSN: 0340-6245
DOI: http://dx.doi.org/10.1160/TH11-11-0804
Issue: 2012: 107/5(May) pp. 803-1007
Pages: 985-997

Impact of dabigatran on a large panel of routine or specific coagulation assays

Laboratory recommendations for monitoring of dabigatran etexilate

See also Editorial by ten Cate

Online Supplementary Material

J. Douxfils (1), F. Mullier (1, 2), S. Robert (1), C. Chatelain (3), B. Chatelain (2), J.-M. Dogné (1)

(1) Department of Pharmacy, Namur Thrombosis and Hemostasis Center (NTHC), Namur Research Institute for LIfe Sciences (NARILIS), University of Namur, Belgium; (2) Hematology Laboratory, Namur Thrombosis and Hemostasis Center (NTHC), Namur Research Institute for LIfe Sciences (NARILIS), CHU Mont-Godinne, Université Catholique de Louvain, Belgium; (3) Hematology Department, Namur Thrombosis and Hemostasis Center, CHU Mont-Godinne, Université Catholique de Louvain, Belgium

Keywords

monitoring, thrombosis, bleeding, dabigatran, Hemoclot Thrombin Inhibitor, new oral anticoagulant

Summary

Due to low bioavailability and high inter-individual variability, monitoring of dabigatran may be required in specific situations to prevent the risk of bleedings or thrombosis. The aim of the study was to determine which coagulation assay(s) could be used to assess the impact of dabigatran on secondary haemostasis. Dabigatran was spiked at concentrations ranging from 4.7 ng/ml to 943.0 ng/ml in pooled citrated human platelet-poor plasma. The following clotting assays were performed: prothrombin time (PT); activated partial thromboplastin time (aPTT); thrombin time (TT); ecarin clotting time (ECT); ecarin chromogenic assay (ECA); prothrombinase-induced clotting time (PiCT); activated clotting time (ACT); Hemoclot Thrombin Inhibitor (HTI) and thrombin generation assay (TGA). A concentration-dependent prolongation of PT, dPT, and aPTT was observed with aPTT being the more sensitive test. The results varied mostly due to the clotting reagent. HTI, ECT and TGA were the most sensitive tests but are not available 24 hours a day. In addition, HTI showed a linear correlation with a good reproducibility. Dabigatran induced a concentration-dependent delay and inhibition of tissue factor-induced TGA. Cut-offs related with higher risk of bleedings or thrombosis were defined for each reagent of aPTT and HTI. In conclusion, aPTT could be used for the monitoring of dabigatran and as screening test for the risk of overdose. However, because of its higher sensitivity, good reproducibility, excellent linear correlation at all doses, its simplicity of use, and possibilities of automation, HTI should be considered as the gold-standard.

You may also be interested in...

1.
S. Moll

Hämostaseologie 2007 27 3: 191-200

2.
Yi-Xin Wang, Valdeci da Cunha, Jon Vincelette, Lei Zhao, Mariko Nagashima, Kohichi Kawai, Shendong Yuan, Kumar Emayan, Imadul Islam, Junko Hosoya, Mark E. Sullivan, William P. Dole, John Morser, Brad O. Buckman, Ronald Vergona

Thromb Haemost 2007 97 1: 54-61

http://dx.doi.org/10.1160/TH06-09-0552

3.

Y. Xu (1), T.-Q. Cai (2), G. Castriota (1), Y. Zhou (2), L. Hoos (2), N. Jochnowitz (2), C. Loewrigkeit (2), J. A. Cook (2), A. Wickham (2), J. M. Metzger (2), M. L. Ogletree (1), D. A. Seiffert (1), Z. Chen (1)

Thromb Haemost 2014 111 4: 694-704

http://dx.doi.org/10.1160/TH13-08-0668