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Platelet genetic biomarker quantification: Comparison of fluorescent microspheres and PCR platforms

Journal: Thrombosis and Haemostasis
ISSN: 0340-6245
Issue: 2013: 109/2 (Feb) pp. 175-360
Pages: 337-346

  1. Stenberg PE, Hill RL. Platelets and megakaryocytes. In: Wintrobe's Clinical Hematology. Philadelphia: Linnincott William Wilkins; 1999. pp. 615-660
  2. Newman P, Gorski J, White G, Gidwitz S, Cretney C, Aster R. Enzymatic amplification of platelet-specific messenger RNA using the polymerase chain reaction. J Clin Invest 1988; 82: 739-743. DOI:10.1172/JCI113656
  3. Levin J. The evolution of mammalian platelets. In: Platelets. Second ed: Elsevier; 2007. pp. 3-23
  4. Fink L, Holschermann H, Kwapiszewska G, et al. Characterization of platelet-specific mRNA by real-time PCR after laser-assisted microdissection. Thromb Haemost. 2003; 90: 749-756.
  5. Rinder H, Schuster J, Rinder C, et al. Correlation of thrombosis with increased platelet turnover in thrombocytosis. Blood 1998; 91: 1288-1294.
  6. Gnatenko DV, Perrotta PL, Bahou WF. Proteomic approaches to dissect platelet function: one-half of the story. Blood 2006; 108: 3983-3991. DOI:10.1182/blood-2006-06-026518
  7. Gnatenko DV, Bahou WF. Recent advances in platelet transcriptomics. Transfus Med Hemother 2006; 33: 217-226. DOI:10.1159/000091371
  8. Dittrich M, Birschmann I, Pfrang J, et al. Analysis of SAGE data in human platelets: features of the transcriptome in an anucleate cell. Thromb Haemost 2006; 95: 643-651.
  9. Gnatenko DV, Dunn JJ, McCorkle SR, et al. Transcript profiling of human platelets using microarray and serial analysis of gene expression. Blood 2003; 101: 2285-2293. DOI:10.1182/blood-2002-09-2797
  10. Bugert P, Dugrillon A, Gunaydin A, et al. Messenger RNA profiling of human platelets by microarray hybridization. Thromb Haemost 2003; 90: 738-748.
  11. McRedmond JP, Park SD, Reilly DF, et al. Integration of proteomics and genomics in platelets: a profile of platelet proteins and platelet-specific genes. Mol Cell Proteomics 2004; 3: 133-144.
  12. Gnatenko DV, Cupit LD, Huang EC, et al. Platelets express steroidogenic 17beta-hydroxysteroid dehydrogenases. Distinct profiles predict the essential thrombocythemic phenotype. Thromb Haemost 2005; 94: 412-421.
  13. Shim MH, Hoover A, Blake N, et al. Gene expression profile of primary human CD34+CD38lo cells differentiating along the megakaryocyte lineage. Exp Hematol 2004; 32: 638-648. DOI:10.1016/j.exphem.2004.04.002
  14. Tenedini E, Fagioli ME, Vianelli N, et al. Gene expression profiling of normal and malignant CD34-derived megakaryocytic cells. Blood 2004; 104: 3126-3135. DOI:10.1182/blood-2003-07-2597
  15. Rowley JW, Oler AJ, Tolley ND, et al. Genome-wide RNA-seq analysis of human and mouse platelet transcriptomes. Blood 2011; 118: e101-111. DOI:10.1182/blood-2011-03-339705
  16. Cecchetti L, Tolley ND, Michetti N, et al. Megakaryocytes differentially sort mRNAs for matrix metalloproteinases and their inhibitors into platelets: a mechanism for regulating synthetic events. Blood 2011; 118: 1903-1911. DOI:10.1182/blood-2010-12-324517
  17. Kieffer N, Guichard J, Farcet JP, et al. Biosynthesis of major platelet proteins in human blood platelets. Eur J Biochem. 1987; 164: 189-195. DOI:10.1111/j.1432-1033.1987.tb11010.x
  18. Weyrich AS, Zimmerman GA. Platelets: signaling cells in the immune continuum. Trends Immunol 2004; 25: 489-495. DOI:10.1016/
  19. Denis MM, Tolley ND, Bunting M, et al. Escaping the nuclear confines: signal-dependent pre-mRNA splicing in anucleate platelets. Cell 2005; 122: 379-391. DOI:10.1016/j.cell.2005.06.015
  20. Senzel L, Gnatenko D, Bahou W. Platelet transcriptome and cardiovascular disease. Future Cardiol 2007; 3: 391-398. DOI:10.2217/14796678.3.4.391
  21. Gnatenko DV, Zhu W, Bahou WF. Multiplexed genetic profiling of human blood platelets using fluorescent microspheres. Thromb Haemost 2008; 100: 929-936.
  22. Gnatenko DV, Zhu W, Xu X, et al. Class prediction models of thrombocytosis using genetic biomarkers. Blood 2010; 115: 7-14. DOI:10.1182/blood-2009-05-224477
  23. Kriegova E, Arakelyan A, Fillerova R, et al. PSMB2 and RPL32 are suitable denominators to normalize gene expression profiles in bronchoalveolar cells. BMC Mol Biol 2008; 9: 69. DOI:10.1186/1471-2199-9-69
  24. de Jonge HJM, Fehrmann RSN, de Bont ESJM, et al. Evidence Based Selection of Housekeeping Genes. PloS one 2007; 2: e898. DOI:10.1371/journal.pone.0000898
  25. Ludbrook J. Linear regression analysis for comparing two measurers or methods of measurement: But which regression? Clin Exp Pharmacol Physiol 2010; 37: 692-699. DOI:10.1111/j.1440-1681.2010.05376.x
  26. Linnet K. Evaluation of regression procedures for methods comparison studies. Clin Chem 1993; 39: 424-432.
  27. Canales RD, Luo Y, Willey JC, et al. Evaluation of DNA microarray results with quantitative gene expression platforms. Nature Biotechnol 2006; 24: 1115-1122. DOI:10.1038/nbt1236
  28. Murphy S, Peterson P, Iland H, et al. Experience of the Polycythemia Vera Study Group with essential thrombocythemia: a final report on diagnostic criteria, survival, and leukemic transition by treatment. Sem Hematol 1997; 34: 29-39.
  29. Freedman JE, Larson MG, Tanriverdi K, et al. Relation of platelet and leukocyte inflammatory transcripts to body mass index in the Framingham heart study. Circulation 2010; 122: 119-129. DOI:10.1161/CIRCULATIONAHA.109.928192
  30. Shipp MA, Ross KN, Tamayo P, et al. Diffuse large B-cell lymphoma outcome prediction by gene-expression profiling and supervised machine learning. Nat Med 2002; 8: 68-74. DOI:10.1038/nm0102-68
  31. Bhattacharjee A, Richards WG, Staunton J, et al. Classification of human lung carcinomas by mRNA expression profiling reveals distinct adenocarcinoma subclasses. Proc Natl Acad Sci USA 2001; 98: 13790-13795. DOI:10.1073/pnas.191502998
  32. Singh D, Febbo PG, Ross K, et al. Gene expression correlates of clinical prostate cancer behavior. Cancer Cell 2002; 1: 203-209. DOI:10.1016/S1535-6108(02)00030-2
  33. Ferrando AA, Neuberg DS, Staunton J, et al. Gene expression signatures define novel oncogenic pathways in T cell acute lymphoblastic leukemia. Cancer Cell 2002; 1: 75-87. DOI:10.1016/S1535-6108(02)00018-1
  34. Ramaswamy S, Ross KN, Lander ES, et al. A molecular signature of metastasis in primary solid tumors. Nat Genet 2003; 33: 49-54. DOI:10.1038/ng1060
  35. Kralovics R, Passamonti F, Buser AS, et al. A gain-of-function mutation of JAK2 in myeloproliferative disorders. N Engl J Med 2005; 352: 1779-1790. DOI:10.1056/NEJMoa051113
  36. Finazzi G, Harrison C. Essential thrombocythemia. Semin Hematol 2005; 42: 230-238. DOI:10.1053/j.seminhematol.2005.05.022
  37. Yang M, Rajan S, Issa AM. Cost effectiveness of gene expression profiling for early stage breast cancer: A decision-analytic model. Cancer 2012; Epub ahead of print

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