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Alternatively spliced human tissue factor (asHTF) is not pro-coagulant

Journal: Thrombosis and Haemostasis
ISSN: 0340-6245
DOI: http://dx.doi.org/10.1160/TH06-09-0524
Issue: 2007: 97/1 (Jan) pp.1-167
Pages: 11-14

Alternatively spliced human tissue factor (asHTF) is not pro-coagulant

Petra Censarek*1, Anett Bobbe*1, Maria Grandoch2, Karsten Schrör1, Artur-Aron Weber1
1Institut für Pharmakologie und Klinische Pharmakologie, Universitätsklinikum Düsseldorf, Germany; 2Institut für Pharmakologie, Universität Duisburg-Essen, Universitätsklinikum, Germany

Keywords

thrombosis, Coagulation factors, tissue factor / factor VII

Summary

It has been proposed that alternatively-spliced human tissue factor (asHTF) is pro-coagulant.We have evaluated the function of asHTF in a mammalian expression system.Full-length human tissue factor (HTF) and asHTF were cloned from smooth muscle cells and over-expressed in HEK293 cells. As expected, a marked pro-coagulant activity (FX activation, thrombin generation) was observed on the surface, in lysates, and on microparticles from HTF transfected cells. In contrast, no pro-coagulant activity of asHTF was observed.

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