Advertisement
Advertisement

Contact Person

Dr. Elinor Switzer

Managing Editor

Phone: +49 (0)711 - 2 29 87 63
Fax: +49 (0)711 - 2 29 87 65
send an Email


Archive

The influence of fibrin polymerization and platelet-mediated contractile forces on citrated whole blood thromboelastography profile

Journal: Thrombosis and Haemostasis
ISSN: 0340-6245
DOI: http://dx.doi.org/10.1160/TH05-07-0500
Issue: 2006: 95/5 (May) pp. 755-916
Pages: 822-828

The influence of fibrin polymerization and platelet-mediated contractile forces on citrated whole blood thromboelastography profile

Tahar Chakroun1 , Grigoris T. Gerotziafas 1 , Jerhad Seghatchian2 , Meyer M. Samama1 , Mohamed Hatmi1,3, Ismail Elalamy 1
1 Service d’Hématologie Biologique Hôtel-Dieu de Paris, Paris, France; 2 Blood component technology and haemostasis / thrombosis consultancy, London, United Kingdom; 3 Département de Médecine Moléculaire, Institut Pasteur Paris, Paris, France

Keywords

Fibrin polymerization, cytochalasin D, platelet-contractility, thromboelastography, TEG ®

Summary

Thromboelastography analysis providing a global assessment of coagulation is gaining new interest in clinical practice.MinimalTF triggered whole blood thromboelastography provides a valuable tool for studying the kinetics of clot formation (expressed by the parameters R, K and α -angle) and the physical characteristics of the clot, such as its firmness and the elastic modulus shear (expressed by the parameters maximal amplitude MA and G).We studied the influence of fibrin polymerization and platelet functional status on each parameter of thromboelastographic trace obtained by minimalTF activation inWB by employing increasing concentrations of a fibrin polymerization inhibitors (the tetrapeptide Gly-Pro-Arg-Pro-OH.AcOH; Pefabloc-FG® ) and an inhibitor of actin polymerization (Cytochalasin D). Pefabloc-FG® at concentrations higher than 5 mg/ml prolonged the R and K times and decreased the α -angle in a concentration-dependent manner but it did not modify MA and G parameters.At the concentration of 5 mg/ml, Pefabloc-FG® completely inhibited clot formation. Cytochalasin D had no effect on R time but decreased the α -angle,MA and G parameters by reaching a plateau at the concentration of 5 µM.The effect of cytochalasin D was more pronounced on MA and G than on the α -angle. A combination of both Pefabloc-FG® (0.5 mg/ml) and cytochalasin D (50 µM) significantly decreased α -angle compared to control as well as their single effect. However, G value was dramatically reduced in the presence of cytochalasin D exposure, without any additional effect when both inhibitors were combined. This study confirms the importance of fibrin polimerization on the kinetics of thrombus formation and demonstrates the close association between the quality of the thrombus and the functional status of platelets. Normal platelet contractile forces are of major importance for the maximum amplitude of TEG which is related to the strength and elastic modulus of the thrombus.

You may also be interested in...

1.
Konstantinos Konstantinidis1, Thomas Gerasimidis1, Elisabeth Verdy2,3, Ismail Elalamy2,3, Michel M. Samama3,4, Grigoris T. Gerotziafas2,3

Thromb Haemost 2007 97 1: 109-118

http://dx.doi.org/10.1160/TH05-10-0701

2.

Online Supplementary Material

J. Rheinlaender (1), S. Vogel (2), J. Seifert (1), M. Schächtele (1), O. Borst (2), F. Lang (3), M. Gawaz (2), T. E. Schäffer (1)

Thromb Haemost 2015 : -

http://dx.doi.org/10.1160/TH14-05-0414

3.

Online Supplementary Material

K. Balasubramaniam (1), G. Viswanathan (1), J. Dragone (2), R. Grose-Hodge (3), P. Martin (2), S. Troy (2), P. Preston (2), A. G. Zaman (1, 4)

Thromb Haemost 2014 112 1: 205-215

http://dx.doi.org/10.1160/TH13-08-0681