Advertisement
Advertisement

Contact Person

Dr. Elinor Switzer

Managing Editor

Phone: +49 (0)711 - 2 29 87 63
Fax: +49 (0)711 - 2 29 87 65
send an Email


Archive

Multiple electrode aggregometry: A new device to measure platelet aggregation in whole blood

Journal: Thrombosis and Haemostasis
ISSN: 0340-6245
DOI: http://dx.doi.org/10.1160/TH06-05-0242
Issue: 2006: 96/6 (Dec) pp. 697-863
Pages: 781-788

Multiple electrode aggregometry: A new device to measure platelet aggregation in whole blood

Orsolya Tóth1,2, Andreas Calatzis3, Sandra Penz1, Hajna Losonczy2, Wolfgang Siess1
1Institute for Prevention of Cardiovascular Diseases, University of Munich, Munich, Germany; 21stDepartment of Medicine, University of Pécs, Pécs, Hungary; 3Department of Transfusion Medicine and Hemostaseology, University of Munich, Munich, Germany

Keywords

anticoagulation, platelet aggregation, multiple electrode aggregometry, single platelet counting

Summary

Several methods are used to analyse platelet function in whole blood. A new device to measure whole blood platelet aggregation has been developed, called multiple electrode platelet aggregometry (MEA).Our aim was to evaluate MEA in comparison with the single platelet counting (SPC) method for the measurement of platelet aggregation and platelet inhibition by aspirin or apyrase in diluted whole blood. Platelet aggregation induced by different concentrations of ADP, collagen and TRAP-6 and platelet inhibition by apyrase or aspirin were determined in citrateor hirudin-anticoagulated blood by MEA and SPC. MEA indicated that spontaneous platelet aggregation was lower, and stimulated platelet aggregation was higher in hirudin- than citrate- anticoagulated blood. In hirudin-anticoagulated, but not citrate- anticoagulated blood, spontaneous platelet aggregation measured by MEA was inhibited by apyrase. For MEA compared with SPC the dose response-curves of agonist-induced platelet aggregation in citrate- and hirudin-blood showed similar EC50 values for TRAP, and higher EC50 values for ADP (non-significant) and collagen (p<0.05).MEA and the SPC method gave similar results concerning platelet-inhibition by apyrase and aspirin. MEA was more sensitive than SPC to the inhibitory effect of aspirin in collagen-induced aggregation. In conclusion, MEA is an easy, reproducible and sensitive method for measuring spontaneous and stimulated platelet aggregation, and evaluating antiplatelet drugs in diluted whole blood.The use of hirudin as an anticoagulant is preferable to the use of citrate.MEA is a promising technique for experimental and clinical applications.

You may also be interested in...

1.
T. Duning1, P. Kirchhof2, S. Knecht1

Nervenheilkunde 2008 27 3: 175-186

2.
A.-A. Weber1, M. Adamzik2, H. S. Bachmann3, K. Görlinger2, M. Grandoch1, K. Leineweber4, H. Müller-Beißenhirtz5, F. Wenzel6, C. Naber7 für die interdisziplinäre Studiengruppe Clinical Pharmacology of Haemostasis

Hämostaseologie 2008 28 1: 66-71

3.
Joseph A. Jakubowski1, Christopher D. Payne2, Ying G. Li1, John T. Brandt1, David S. Small1, Nagy A. Farid1, Daniel E. Salazar3, Kenneth J. Winters1

Thromb Haemost 2008 99 2: 409-415

http://dx.doi.org/10.1160/TH07-09-0575