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Chrono-lume ® and Magnesium Potentiate Aggregation of Canine but not Human Platelets in Citrated Platelet-Rich Plasma

Journal: Thrombosis and Haemostasis
ISSN: 0340-6245
Issue: 1998: 80/1 (July) pp.1-210
Pages: 176-180
Ahead of Print: ###MANUSCRIPT_aheadofprint###

Chrono-lume ® and Magnesium Potentiate Aggregation of Canine but not Human Platelets in Citrated Platelet-Rich Plasma

Mary Beth Callan (1) , Frances S. Shofer (1) , Carol Wojenski (2) , Urs Giger (1)
From the (1) Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, and the (2) Cardeza Foundation and Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson Medical College, Philade

Summary

The effects of Chrono-lume ® (CL) and magnesium sulfate (Mg 2+ ), a component of this luciferin-luciferase reagent, on platelet aggregation were studied in platelet-rich plasma (PRP) obtained from blood anti-coagulated with sodium citrate from humans, dogs, cats, horses, and cows. The final added Mg 2+ concentration of both solutions ranged from 0.75-3.7 mM. CL and Mg 2+ had no effect on maximum aggregation of platelets from humans induced by sub-threshold concentrations of collagen and ADP. In contrast, addition of CL or Mg 2+ to canine PRP resulted in a dose-dependent and equal potentiation of platelet aggregation in response to sub-threshold concentrations of collagen, ADP, and thrombin in normal and thrombopathic dogs. The effect of CL on platelet aggregation induced by sub-threshold concentrations of agonists was less pronounced and varied in other species according to the agonist. The reason for the marked difference in sensitivity of human and canine platelets to CL or Mg 2+ is not clear, although a difference in releasable cation pools of the platelets from these two species has been recognized. Platelet aggregation studies of animals with suspected thrombopathias should be performed without CL to prevent masking of a platelet function defect.