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Effects of tissue factor, thrombomodulin and elevated clotting factor levels on thrombin generation in the calibrated automated thrombogram

Journal: Thrombosis and Haemostasis
ISSN: 0340-6245
Topic:

Heparin and its derivatives – Present and future

DOI: http://dx.doi.org/10.1160/TH09-03-0180
Issue: 2009: 102/5 (Nov) pp. 799-1006
Pages: 936-944

Effects of tissue factor, thrombomodulin and elevated clotting factor levels on thrombin generation in the calibrated automated thrombogram

Kellie R. Machlus1; Emily A. Colby2; Jogin R. Wu3; Gary G. Koch2; Nigel S. Key4; Alisa S. Wolberg1

1Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA; 2Department of Biostatistics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA; 3Department of Pathology, Duke University Medical Center, Durham, North Carolina, USA; 4Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA

Keywords

thrombosis, Platelets, Tissue factor, thrombin, hypercoagulability, thrombomodulin

Summary

Elevated procoagulant levels have been correlated with increased thrombin generation in vitro and with increased venous thromboembolism (VTE) risk in epidemiological studies. Thrombin generation tests are increasingly being employed as a high throughput method to provide a global measure of procoagulant activity in plasma samples. The objective of this study was to distinguish the effects of assay conditions [tissue factor (TF), thrombomodulin, platelets/lipids] and factor levels on thrombin generation parameters, and determine the conditions and parameters with the highest sensitivity and specificity for detecting elevated factor levels. Thrombin generation was measured using calibrated automated thrombography (CAT) in corn trypsin inhibitor (CTI)-treated platelet-free plasma (PFP) and plateletrich plasma (PRP). Statistical analysis was performed using logarithms of observed values with analysis of variance that ac- counted for experiment and treatment. The relative sensitivity of lag time (LT), time to peak (TTP), peak height and endogenous thrombin potential (ETP) to elevated factors XI, IX, VIII, X, and prothrombin was as follows: PFP initiated with 1 pM TF > PFP initiated with 5 pM TF > PRP initiated with 1 pM TF. For all conditions, inclusion of thrombomodulin prolonged the LT and decreased the peak and ETP; however, addition of thrombomodulin did not increase the ability of CAT to detect elevated levels of individual procoagulant factors. In conclusion, CAT conditions differentially affected the sensitivity of thrombin generation to elevated factor levels. Monitoring the peak height and/ or ETP following initiation of clotting in PFP with 1 pM TF was most likely to detect hypercoagulability due to increased procoagulant factor levels.

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