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Proteome of human plasma very low-density lipoprotein and low-density lipoprotein exhibits a link with coagulation and lipid metabolism

Journal: Thrombosis and Haemostasis
ISSN: 0340-6245
DOI: http://dx.doi.org/10.1160/TH13-02-0178
Issue: 2014: 111/3 (Mar) pp. 381-564
Pages: 518-530

Proteome of human plasma very low-density lipoprotein and low-density lipoprotein exhibits a link with coagulation and lipid metabolism

Online Supplementary Material

M. Dashty (1, 2), M. M. Motazacker (3), J. Levels (3), M. de Vries (1), M. Mahmoudi (4, 5), M. P. Peppelenbosch (2), F. Rezaee (1, 2)

(1) Department of Cell Biology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands; (2) Department of Gastroenterology and Hepatology, Erasmus Medical Center, Rotterdam, The Netherlands; (3) Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands; (4) Nanotechnology Research, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; (5) Current Address: Department of Pediatrics, Division of Cardiology, School of Medicine, Stanford University, Stanford, California, USA

Keywords

proteomics, coagulation, prothrombin, protein S, atherothrombosis, CETP, Apolipoproteins, nLC-MS/MS, TFPI-1, LCAT, PLTP, apoAV, anti-microbial peptides, lysozyme C, dermicidin, lipopolysaccharide binding protein, CD14, prenylcysteine oxidase, cathepcin D

Summary

Apart from transporting lipids through the body, the human plasma lipoproteins very low-density lipoprotein (VLDL) and low-density lipoprotein (LDL) are also thought to serve as a modality for intra-organismal protein transfer, shipping proteins with important roles in inflammation and thrombosis from the site of synthesis to effector locations. To better understand the role of VLDL and LDL in the transport of proteins, we applied a combination of LTQ ORBITRAP-XL (nLC-MS/MS) with both in-SDS-PAGE gel and in-solution tryptic digestion of pure and defined VLDL and LDL fractions. We identified the presence of 95 VLDL- and 51 LDL-associated proteins including all known apolipoproteins and lipid transport proteins, and intriguingly a set of coagulation proteins, complement system and anti- microbial proteins. Prothrombin, protein S, fibrinogen γ, PLTP, CETP, CD14 and LBP were present on VLDL but not on LDL. Prenylcysteine oxidase 1, dermcidin, cathelicidin antimicrobial peptide, TFPI-1 and fibrinogen α chain were associated with both VLDL and LDL. Apo A-V is only present on VLDL and not on LDL. Collectively, this study provides a wealth of knowledge on the protein constituents of the human plasma lipoprotein system and strongly supports the notion that protein shuttling through this system is involved in the regulation of biological processes. Human diseases related to proteins carried by VLDL and LDL can be divided in three major categories: 1 – dyslipidaemia, 2 – atherosclerosis and vascular disease, and 3 – coagulation disorders.

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