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The role of reverse genetics systems in studying viral hemorrhagic fevers

Journal: Thrombosis and Haemostasis
ISSN: 0340-6245

Vascular Endothelium and Infectious Diseases

Issue: 2005: 94/2 (Aug) pp. 233-468
Pages: 240-253

The role of reverse genetics systems in studying viral hemorrhagic fevers

Hideki Ebihara1,5, Allison Groseth 2,3, Gabriele Neumann 4, Yoshihiro Kawaoka1,4,5, Heinz Feldmann2,3
1 Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan 2National Microbiology Laboratory, National Laboratory for Zoonotic Diseases and Special Pathogens, Public Health Agency of


Viral hemorrhagic fever (VHF) is an infectious syndrome in humans often associated with high fatality rates. For most VHFs there are no specific and effective therapies or vaccines available and, in general, there is a lack of knowledge regarding the biology and pathogenesis of the causative agents.Therefore, a more detailed understanding of the molecular basis ofVHF pathogenesis, including the identification of viral virulence determinants and host interactions and responses, will be important to en hance our ability to control VHF infections.The recently developed “reverse genetics systems” for severalVHF causing viruses have allowed the generation of infectious viruses from cloned cDNA and thus, the generation of virus mutants. Here we review the existing reverse genetics systems for VHF causing viruses and discuss their use in studying viral replication, pathogenesis, and the development of antivirals and vaccines.