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CD14+CD16+ monocytes in coronary artery disease and their relationship to serum TNF-α levels

Journal: Thrombosis and Haemostasis
ISSN: 0340-6245

Inflammation and Wound Healing

Issue: 2004: 92/2 (Aug) pp. 227-434
Pages: 419-424

CD14+CD16+ monocytes in coronary artery disease and their relationship to serum TNF-α levels

Axel Schlitt(1*), Gunnar H. Heine(2*), Stefan Blankenberg(1), Christine Espinola-Klein(1), Joern F. Dopheide(1), Christoph Bickel(1), Karl J. Lackner(1), Mete Iz(1), Juergen Meyer(1), Harald Darius(1), Hans J. Rupprecht(1)
(1)Department of Medicine II and Institute of Clinical Chemistry and Laboratory Medicine, Johannes Gutenberg University, Mainz,Germany (2)Department of Medicine IV, University of the Saarland, Homburg/Saar, Germany


Monocytes play a central role in the inflammatory disease atherosclerosis. CD14+CD16+ monocytes are considered proinflammatory monocytes, as they have an increased capacity to produce proinflammatory cytokines, such as TNF-α, and are elevated in various inflammatory diseases.We hypothesized that patients with coronary artery disease (CAD) have increased levels of CD14+CD16+ monocytes, and that CD14+CD16+ monocytes are associated with inflammation markers. We investigated CD14+CD16+ monocytes in 247 patients with CAD and 61 control subjects using flow cytometry. In addition serum concentrations of TNF-α, IL-6, and Hs-CRP were assessed. Patients with CAD had higher levels of CD14+CD16+ monocytes than controls (13.6% versus 11.4%; p<0.001). Logistic regression analysis including quartiles of CD14+CD16+ monocytes showed that CD14+CD16+ monocytes were associated with prevalence of CAD (OR 4.9, 95% CI 2.5–19.1, for subjects in the fourth quartile in comparison to subjects in the first quartile). The association between CD14+CD16+ monocytes and CAD remained independently significant after adjustment for most potential confounders (OR 5.0, 95% CI 1.2-20.0). Serum concentrations of TNF-α were elevated in subjects within the highest quartiles of CD14+CD16+ monocytes (p=0.018). Our study showed that increased numbers of CD14+CD16+ monocytes are associated with coronary atherosclerosis and TNF-α. In accordance, recent animal studies suggest a possibly important role of these monocytes in the development of atherosclerosis.