Advertisement
Advertisement

Archive

Cooperation between human fibrocytes and endothelial colony-forming cells increases angiogenesis via the CXCR4 pathway

Journal: Thrombosis and Haemostasis
ISSN: 0340-6245
Topic:

Theme Issue
Coagulation proteases and cardiovascular disease

DOI: https://doi.org/10.1160/TH13-08-0711
Issue: 2014: 112/5 (Nov) pp. 843–1075
Pages: 1002-1013

Cooperation between human fibrocytes and endothelial colony-forming cells increases angiogenesis via the CXCR4 pathway

Online Supplementary Material

D. M. Smadja (1, 2, 3, 4), P. Dorfmüller (5, 6), C. L. Guerin (2, 4, 7), I. Bieche (2, 8), C. Badoual (2, 7, 9), E. Boscolo (10), M. Kambouchner (11), A. Cazes (2, 9), O. Mercier (5, 6, 12), M. Humbert (5, 6, 13), P. Gaussem (2, 3, 4), J. Bischoff (1), D. Israël-Biet (2, 4, 14)

(1) Vascular Biology Program and Surgery Department, Children`s Hospital, Harvard Medical School, Boston, Massachusetts, USA; (2) Université Paris Descartes, Sorbonne Paris Cite, Paris, France; (3) AP-HP, Hôpital Européen Georges Pompidou, Service d`Hématologie Biologique, Paris, France; (4) Inserm UMR-S1140, Paris, France; (5) Univ. Paris-Sud, Faculté de Médecine, Le Kremlin-Bicêtre, France; (6) Inserm UMR-S999, LabEx LERMIT, Centre Chirurgical Marie Lannelongue, Le Plessis Robinson, France; (7) PARCC, Inserm UMR-S970, Paris, France; (8) Inserm UMR-S745, Paris, France; (9) AP-HP, Hôpital Européen Georges Pompidou, Service d`anatomopathologie, Paris, France; (10) Experimental Hematology and Cancer Biology, Cancer and Blood Disease Institute, Cincinnati Children’s Hospital Medical Center, Cincinnati, Ohio, USA; (11) AP-HP, Hôpital Avicenne, Service d`anatomopathologie, Bobigny, France; (12) Service de chirurgie thoracique, Centre Chirurgical Marie Lannelongue, Le Plessis Robinson, France; (13) AP-HP, Hôpital Bicêtre, Service de Pneumologie, DHU Thorax Innovation (TORINO), Le Kremlin-Bicêtre, France; (14) AP-HP, Hôpital Européen Georges Pompidou, Service de Pneumologie, Centre de compétences Maladies rares pulmonaires, Paris, France

Keywords

endothelial progenitor cells, CXCR4, pulmonary fibrosis, Fibrocytes, ECFC

Summary

Fibrotic diseases of the lung are associated with a vascular remodelling process. Fibrocytes (Fy) are a distinct population of blood-borne cells that co-express haematopoietic cell antigens and fibroblast markers, and have been shown to contribute to organ fibrosis. The purpose of this study was to determine whether fibrocytes cooperate with endothelial colony-forming cells (ECFC) to induce angiogenesis. We isolated fibrocytes from blood of patient with idiopathic pulmonary fibrosis (IPF) and characterised them by flow cytometry, quantitative reverse transcriptase PCR (RTQ-PCR), and confocal microscopy. We then investigated the angiogenic interaction between fibrocytes and cord-blood-derived ECFC, both in vitro and in an in vivo Matrigel implant model. Compared to fibroblast culture medium, fibrocyte culture medium increased ECFC proliferation and differentiation via the SDF-1/CXCR4 pathway. IPF-Fy co-implanted with human ECFC in Matrigel plugs in immunodeficient mice formed functional microvascular beds, whereas fibroblasts did not. Evaluation of implants after two weeks revealed an extensive network of erythrocyte-containing blood vessels. CXCR4 blockade significantly inhibited this blood vessel formation. The clinical relevance of these data was confirmed by strong CXCR4 expression in vessels close to fibrotic areas in biopsy specimens from patients with IPF, by comparison with control lungs. In conclusion, circulating fibrocytes might contribute to the intense remodelling of the pulmonary vasculature in patients with idiopathic pulmonary fibrosis.

You may also be interested in...

1.

E. Shantsila (1), L. D. Tapp (1), B. J. Wrigley (1), S. Montoro-García (1), G. Y. H. Lip (1)

Thromb Haemost 2013 109 2: 255-262

https://doi.org/10.1160/TH12-06-0395

2.
Colin Gerard Egan 1,2; Francesca Caporali 1,2; Alda F. Huqi 3,4; Maria Cristina Zito 3; Marta Focardi 3; Sergio Mondillo 3; Carlo Pierli 3; Mario Marzilli 4; Vincenzo Sorrentino 1,2

Thromb Haemost 2009 101 6: 1138-1146

https://doi.org/10.1160/TH08-11-0723

3.

Konstantinos Stellos, Meinrad Gawaz

Thromb Haemost 2007 98 5: 922-929

https://doi.org/10.1160/TH07-02-0147